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- Title
Physical mapping of puroindoline b- 2 genes and molecular characterization of a novel variant in durum wheat ( Triticum turgidum L.).
- Authors
Chen, F.; Xu, H.-X.; Zhang, F.-Y.; Xia, X.-C.; He, Z.-H.; Wang, D.-W.; Dong, Z.-D.; Zhan, K.-H.; Cheng, X.-Y.; Cui, D.-Q.
- Abstract
The puroindoline genes ( Pina and Pinb) are the functional components of the common or bread wheat ( Triticum aestivum L.) grain hardness locus that are responsible for kernel texture. In this study, four puroindoline b- 2 variants were physically mapped using nulli-tetrosomic lines of bread wheat cultivar Chinese Spring and substitution lines of durum wheat ( Triticum turgidum L.) cultivar Langdon. Results indicated that Pinb- 2v1 was on 7D of Chinese Spring, Pinb- 2v2 on 7B of Chinese Spring, Pinb- 2v3 on 7B of Chinese Spring and Langdon, and Pinb- 2v4 on 7A of Chinese Spring and Langdon. A new puroindoline b- 2 variant, designated Pinb- 2v5, was identified at the puroindoline b- 2 locus of durum wheat cultivar Langdon, with a difference of only five single nucelotide polymorphisms compared with Pinb- 2v4. Sequencing results indicated that, in comparison with the Pinb- 2v3 sequence (AM99733 and GQ496618 with one base-pair modification of G to T at 6th position, designated Pinb- 2v3a) in bread wheat cultivar Witchta, the coding region of Pinb- 2v3 in 12 durum wheat cultivars had a single nucleotide change from T to C at the 311th position, resulting in a corresponding amino acid change from valine to alanine at the 104th position. This new allele was designated Pinb- 2v3b. The study of puroindoline b- 2 gene polymorphism in CIMMYT and Italian durum wheat germplasm and discovery of a novel puroindoline b- 2 variant could provide useful information for further understanding the molecular and genetic basis of kernel hardness and illustrating gene duplication events in wheat.
- Subjects
DURUM wheat; WHEAT genetics; PLANT gene mapping; PLANT molecular genetics; GENETIC polymorphisms; NUCLEOTIDE sequence; PLANT germplasm
- Publication
Molecular Breeding, 2011, Vol 28, Issue 2, p153
- ISSN
1380-3743
- Publication type
Article
- DOI
10.1007/s11032-010-9469-2