We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Transferosomes stabilized hydrogel incorporated rhodomyrtone-rich extract from Rhodomyrtus tomentosa leaf fortified with phosphatidylcholine for the management of skin and soft-tissue infections.
- Authors
Ontong, Julalak Chorachoo; Singh, Sudarshan; Siriyong, Thanyaluck; Voravuthikunchai, Supayang P.
- Abstract
Rhodomyrtus tomentosa leaf (RT)-incorporated transferosomes were developed with lecithin and cholesterol blends with edge activators at different ratios. RT-transferosomes were characterized and employed in transferosomal gel formulations for the management of skin and soft-tissue infections. The optimized formulation entrapped up to 81.90 ± 0.31% of RT with spherical vesicles (405.3 ± 2.0 nm), polydispersity index value of 0.16 ± 0.08, and zeta potential of − 61.62 ± 0.86 mV. Total phenolic and flavonoid contents of RT-transferosomes were 15.65 ± 0.04 μg GAE/g extract and 43.13 ± 0.91 μg QE/g extract, respectively. RT-transferosomes demonstrated minimum inhibitory and minimum bactericidal concentrations at 8–256 and 64–1024 μg/mL, respectively. Free radical scavenging assay showed RT-transferosomes with high scavenging activity against DPPH and ABTS radicals. Moreover, RT-transferosomes demonstrated moderate activity against mushroom tyrosinase, with IC50 values of 245.32 ± 1.32 μg/mL. The biocompatibility results against L929 fibroblast and Vero cells demonstrated IC50 at 7.05 ± 0.17 and 4.73 ± 0.13 μg/mL, respectively. In addition, nitric oxide production significantly decreased by 6.78–88.25% following the treatment with 31.2–500 ng/mL RT-transferosomes (p < 0.001). Furthermore, the freeze–thaw stability study displayed no significant change in stability in the sedimentation and pH of gel fortified with RT-transferosomes. The results suggested that RT-transferosome formulation can be effectively employed as natural biomedicines for scar prevention and the management of skin soft-tissue infections.
- Subjects
SKIN infections; LECITHIN; ZETA potential; FREE radicals; FLAVONOIDS; PHENOL oxidase
- Publication
Biotechnology Letters, 2024, Vol 46, Issue 1, p127
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-023-03452-1