We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Current status and future possibilities of molecular genetics techniques in <italic>Brassica napus</italic>.
- Authors
Afzal, Muhammad; Alghamdi, Salem Safer; Habib ur Rahman, Muhammad; Ahmad, Awais; Farooq, Tahir; Alam, Mukhtar; Khan, Imtiaz Ali; Ullah, Hidayat; Nasim, Wajid; Fahad, Shah
- Abstract
As PCR methods have improved over the last 15 years, there has been an upsurge in the number of new DNA marker tools, which has allowed the generation of high-density molecular maps for all the key Brassica crop types. Biotechnology and molecular plant breeding have emerged as a significant tool for molecular understanding that led to a significant crop improvement in the <italic>Brassica napus</italic> species. <italic>Brassica napus</italic> possess a very complicated polyploidy-based genomics. The quantitative trait locus (QTL) is not sufficient to develop effective markers for trait introgression. In the coming years, the molecular marker techniques will be more effective to determine the whole genome impairing desired traits. Available genetic markers using the single-nucleotide sequence (SNP) technique and high-throughput sequencing are effective in determining the maps and genome polymorphisms amongst candidate genes and allele interactions. High-throughput sequencing and gene mapping techniques are involved in discovering new alleles and gene pairs, serving as a bridge between the gene map and genome evaluation. The decreasing cost for DNA sequencing will help in discovering full genome sequences with less resources and time. This review describes (1) the current use of integrated approaches, such as molecular marker technologies, to determine genome arrangements and interspecific outcomes combined with cost-effective genomes to increase the efficiency in prognostic breeding efforts. (2) It also focused on functional genomics, proteomics and field-based breeding practices to achieve insight into the genetics underlying both simple and complex traits in canola.
- Subjects
BRASSICA; MOLECULAR genetics; PLANT breeding; POLYMERASE chain reaction; PLANT biotechnology; GENETIC markers in plants
- Publication
Biotechnology Letters, 2018, Vol 40, Issue 3, p479
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-018-2510-y