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- Title
Production of conjugated linoleic acid by heterologous expression of linoleic acid isomerase in oleaginous fungus Mortierella alpina.
- Authors
Hao, Danhui; Chen, Haiqin; Hao, Guangfei; Yang, Bo; Zhang, Baixi; Zhang, Hao; Chen, Wei; Chen, Yong
- Abstract
Objective: To increase the commercial value of oleaginous fungus Mortierella alpina by incorporation of trans-10, cis-12 conjugated linoleic acid (CLA) into the polyunsaturated fatty acids (PUFAs) of M. alpina via Propionibacterium acnes isomerase (PAI) conversion. Results: The PAI gene and the codon optimization version were heterologously expressed in M. alpina via Agrobacterium tumefaciens-mediated transformation (ATMT). Coding usage modification significantly improved the translation of PAI transcripts and trans-10, cis-12 CLA was produced up to 1.2 mg l, which corresponds to approx. 0.05 % of the total fatty acid (TFA). Since PAI prefers free linoleic acid as a substrate rather than any other forms, 5 μM long-chain acyl CoA synthetase inhibitor was added and the trans-10, cis-12 CLA content increased approx. 24-fold to 29 mg l, reaching up to 1.2 % (w/w) of the TFA in M. alpina. Conclusion: Heterologous expression of PAI in M. alpina by ATMT methods is a practicable way in biosynthesis of CLA and this system may be a feasible platform for industrial production of CLA.
- Subjects
BIOTECHNOLOGY research; CONJUGATED linoleic acid; UNSATURATED fatty acids; CUTIBACTERIUM acnes; ISOMERASES; AGROBACTERIUM tumefaciens
- Publication
Biotechnology Letters, 2015, Vol 37, Issue 10, p1983
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-015-1871-8