We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Coexpression of multiple genes reconstitutes two pathways of very long-chain polyunsaturated fatty acid biosynthesis in Pichia pastoris.
- Authors
Kim, Sun; Roh, Kyung; Kim, Kwang-Soo; Kim, Hyun; Lee, Kyeong-Ryeol; Kang, Han-Chul; Kim, Jong-Bum
- Abstract
The introduction of novel traits to cells often requires the stable coexpression of multiple genes within the same cell. Herein, we report that C very long-chain polyunsaturated fatty acids (VLC-PUFAs) were synthesized from C precursors by reactions catalyzed by delta 6-desaturase, an ELOVL5 involved in VLC-PUFA elongation, and delta 5-desaturase. The coexpression of McD6DES, AsELOVL5, and PtD5DES encoding the corresponding enzymes, produced docosatetraenoic acid (C n-6) and docosapentaenoic acid (C n-3), as well as arachidonic acid (C n-6) and eicosapentaenoic acid (C n-3) in the methylotrophic yeast Pichia pastoris. The expression of each gene increased within 24 h, with high transcript levels after induction with 0.5 or 1 % methanol. High levels of the newly expressed VLC-PUFAs occurred after 144 h. This expression system exemplifies the recent progress and future possibilities of the metabolic engineering of VLC-PUFAs in oilseed crops.
- Subjects
UNSATURATED fatty acids; BIOSYNTHESIS; DESATURASES; ARACHIDONIC acid; FUNGAL gene expression; PICHIA pastoris
- Publication
Biotechnology Letters, 2014, Vol 36, Issue 9, p1843
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1007/s10529-014-1550-1