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- Title
Changes in Growth, Morphology, and Physiology of Tetrahymena pyriformis Exposed to Continuous Cesium-137 and Cobalt-60 Gamma-Radiation.
- Authors
Ziyadi, Soukaina; Iddar, Abdelghani; Kabine, Mostafa; El Mzibri, Mohammed; Moutaouakkil, Adnane
- Abstract
This study investigated the effects of gamma-radiation on Tetrahymena pyriformis. The experimental approach consists of exposing T. pyriformis growing in presence of Cesium-137 (137Cs) at dose rates of 1, 2, 4, and 6 cGy h−1 and Cobalt-60 (60Co) at dose rates of 8, 10, 15, and 20 cGy h−1. The radiation doses effects on growth, morphology, some metabolic enzymes, and reactive oxygen species (ROS) markers have been evaluated. When cells were growing in irradiating conditions at dose rates beyond 4 cGy h−1, a decreasing of cells and generation numbers with a prolongation of generation time and a change of morphological aspect with rounding-off of cells were observed compared to the control. The 50%-inhibitory dose (ID50) for radiation was estimated at 1568.72 ± 158.45 cGy. The gamma-radiation at dose rates more than 6 cGy h−1, affected both glyceraldehyde 3-phosphate dehydrogenase and succinate dehydrogenase by inhibiting their activities. All of these effects were more pronounced when cells were irradiated at the dose rate of 20 cGy h−1 using 60Co source. For ROS markers generated by gamma-radiation in T. pyriformis, the results showed an increase of the lipid peroxidation in cells grown in presence of gamma-radiation at dose rates more than 6 cGy h−1 and an enhancement in catalase and superoxide dismutase activities from the dose rate of 1 cGy h−1. These encouraging results suggested the use of T. pyriformis as a unicellular model cell to investigate other aspects of the response to ionizing radiation.
- Subjects
TETRAHYMENA pyriformis; SUCCINATE dehydrogenase; GLYCERALDEHYDEPHOSPHATE dehydrogenase; IONIZING radiation; CATALASE; SUPEROXIDE dismutase
- Publication
Current Microbiology, 2022, Vol 79, Issue 2, p1
- ISSN
0343-8651
- Publication type
Article
- DOI
10.1007/s00284-021-02684-6