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- Title
DNA Sequences of the Immunoglobulin Heavy Chain Variable Region Gene in Pyothorax-Associated Lymphoma.
- Authors
Miwa, Hideaki; Takakuwa, Tetsuya; Nakatsuka, Shin-ichi; Tomita, Yasuhiko; Iuchi, Keiji; Aozasa, Katsuyuki
- Abstract
B cell lymphoma develops in the pleural cavity of patients affected by long-standing pyothorax resulting from lung tuberculosis, thus termed pyothorax-associated lymphoma (PAL). PAL usually shows a diffuse large cell morphology, and constantly contains Epstein-Barr virus (EBV) genome. To investigate whether PAL cells proliferate in response to specific antigenic stimuli and its stage in B cell differentiation, immunoglobulin heavy chain gene in 7 cases and 2 cell lines from PAL, all confirmed by histological studies to be EBV-positive diffuse large B cell lymphoma, were examined by using polymerase chain reaction (PCR) method. Clonal rearrangement of the gene was detected in 4 cases of PAL tissues and one cell line. As for the usage of the V region gene (V[sub H] ), the V[sub H] 3 family gene was used in 3 of these 5 cases with different homologous germlines, suggesting that the origin of PAL cells from a repertoire of B lymphocytes responsive to specific antigenic epitope was unlikely. Compared to the homologous germline, the mutation frequency of PAL was 9% on average. Only one case might have more replacement mutations in the complementarity-determining regions than expected by chance, thus antigen-selected maturation might not take place in PAL. Intraclonal sequence heterogeneity in the V[sub H] gene was found in another case. From these findings, it is concluded that PAL is composed of B lymphocytes at the differentiation stage of the postgerminal center. Antigen-selected maturation might not take place in PAL, which is distinct from the majority of B cell lymphomas.Copyright © 2002 S. Karger AG, Basel
- Subjects
IMMUNOGLOBULINS; NUCLEOTIDE sequence; B cells; LYMPHOMAS; RETICULOENDOTHELIAL granulomas
- Publication
Oncology, 2002, Vol 62, Issue 3, p241
- ISSN
0030-2414
- Publication type
Article
- DOI
10.1159/000059572