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- Title
Direct estimation of frequency of cytotoxic T lymphocytes by a modified plaque assay.
- Authors
BONAVIDA, BENJAMIN; IKEJIRI, BARBARA; KEDAR, ELI
- Abstract
THYMUS-DERIVED lymphocytes (T) have been shown to have a major role in cell-mediated immunity against foreign transplants and syngeneic tumours1,2. In vitro, several assays have been developed to measure thymus-dependent cell-mediated immunity, making use of the cytotoxic potential of these effector cells3. Precise estimates of the number of cytotoxic T lymphocytes (CTL), however, are not possible by available methods. We report here a new method to quantify the CTL present in a mixed cell population. The method is based on the ability of CTL to kill specifically adjacent target cells fixed on monolayers and to form zones of lysis or plaques.
- Publication
Nature, 1976, Vol 263, Issue 5580, p769
- ISSN
0028-0836
- Publication type
Article
- DOI
10.1038/263769a0