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- Title
Sponge-Cell Culture? A Molecular Identification Method for Sponge Cells.
- Authors
Detmer Sipkema; Hans G. H. J. Heilig, Takeshi; Antoon D. L. Akkermans, Takeshi; Ronald Osinga; Johannes Tramper; René H. Wijifels, Takeshi
- Abstract
Dissociated sponge cells are easily confused with unicellular organisms. This has been an obstacle in the development of sponge-cell lines. We developed a molecular detection method to identify cells of the sponge Dysidea avara in dissociated cell cultures. The 18S ribosomal RNA gene from a Dysidea avara specimen was sequenced and compared to eukaryotic 18S rDNA sequences picked up from a proliferating cell culture that originated from a dissociated Dysidea avara specimen. Our method proved unambiguously that this was not a sponge-cell culture. Therefore, it provides a valuable tool for further research on sponge-cell cultures.
- Subjects
SPONGES (Invertebrates); CELL culture; MOLECULAR genetics; RNA; NUCLEOTIDE sequence; EUKARYOTIC cells
- Publication
Marine Biotechnology, 2003, Vol 5, Issue 5, p443
- ISSN
1436-2228
- Publication type
Article
- DOI
10.1007/s10126-002-0090-1