We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effects of ghrelin on homocysteine-induced dysfunction and inflammatory response in rat cardiac microvascular endothelial cells.
- Authors
Wang, Dongjuan; Wang, Haichang; Luo, Peng; Hwang, Andrew; Sun, Dongdong; Wang, Yabin; Zhang, Zheng; Liu, Nan; Wang, Shenxu; Li, Chengxiang; Cao, Feng
- Abstract
Ghrelin is a well-characterized hormone that has protective effects on endothelial cells. Elevated HCY (homocysteine) can be a cardiovascular risk factor, but it is not known whether ghrelin can inhibit HCY-induced dysfunction and inflammatory response in rat CMECs (cardiac microvascular endothelial cells). We found that HCY treatment for 24 h inhibited proliferation and NO (nitric oxide) secretion, but with increased cell apoptosis and secretion of cytokines in CMECs. In contrast, ghrelin pretreatment significantly improved proliferation and NO secretion, and inhibited cell apoptosis and secretion of cytokines in HCY-induced CMECs. In addition, Western blot assay showed that NF-κB (nuclear factor κB) and cleaved-caspase 3 expression were elevated, and PCNA (proliferating cell nuclear antigen) and eNOS (endothelial nitric oxide synthase) expression were decreased after treatment with HCY, which was significantly reversed by pretreatment with ghrelin. The data suggest that ghrelin inhibits HCY-induced CMEC dysfunction and inflammatory response, probably mediated by inhibition of NF-κB activation.
- Subjects
GHRELIN; HOMOCYSTEINE; INFLAMMATION; LABORATORY rats; HEART cells; MICROCIRCULATION disorders; ENDOTHELIAL cells
- Publication
Cell Biology International, 2012, Vol 36, Issue 6, p511
- ISSN
1065-6995
- Publication type
Article
- DOI
10.1042/CBI20110235