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- Title
Soluble Adhesion Molecules Correlate with Surface Expression in an In Vitro Model of Endothelial Activation.
- Authors
Kjærgaard, Anders G.; Dige, Anders; Krog, Jan; Tønnesen, Else; Wogensen, Lise
- Abstract
Endothelial activation is a pivotal event in the development and progression of inflammation. Central to endothelial activation is the up-regulation of cellular adhesion molecules (CAMs) including E-selectin (CD62E), ICAM-1 (CD54), VCAM-1 (CD106) and PECAM-1 (CD31). These CAMs are also found in soluble forms ( sCAMs). In this in vitro study of endothelial activation, we examined whether the levels of sCAMs correlate with the endothelial surface expression of CAMs in a dose-dependent and time-dependent manner. Such a correlation would support the use of sCAMs as surrogate markers for endothelial activation in inflammatory conditions. Human umbilical vein endothelial cells (HUVEC) were cultured with various concentrations of TNF-α for 8 hr and at a fixed concentration of TNF-α for various durations. The levels of soluble and surface-bound E-selectin, ICAM-1, VCAM-1 and PECAM-1 were quantified by flow cytometry. TNF-α stimulation increased CAM and sCAM expression in a dose-dependent and time-dependent manner. There was a significant positive correlation between the levels of ICAM-1 and sICAM-1 and between the levels of VCAM and sVCAM-1 in both the dose-response and time-response experiments. A positive correlation between the levels of E-selectin and sE-selectin was observed in the time-response experiment. This study supports the use of sCAMs as potential biomarkers of endothelial activation. In particular, the use of sICAM-1, sVCAM-1 and sE-selectin seems promising.
- Subjects
CELL adhesion molecules; ENDOTHELIAL cells; UMBILICAL veins; FLOW cytometry; BIOMARKERS; CYTOKINES
- Publication
Basic & Clinical Pharmacology & Toxicology, 2013, Vol 113, Issue 4, p273
- ISSN
1742-7835
- Publication type
Article
- DOI
10.1111/bcpt.12091