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- Title
Glanders Diagnosis in an Asymptomatic Mare from Brazil: Insights from Serology, Microbiological Culture, Mass Spectrometry, and Genome Sequencing.
- Authors
Suniga, Paula Adas Pereira; Mantovani, Cynthia; dos Santos, Maria Goretti; do Egito, Andréa Alves; Verbisck, Newton Valério; dos Santos, Lenita Ramires; Dávila, Alberto Martín Rivera; Zimpel, Cristina Kraemer; Zerpa, Maria Carolina Sisco; Chiebao, Daniela Pontes; de Sá Guimarães, Ana Márcia; de Castro Nassar, Alessandra Figueiredo; de Araújo, Flábio Ribeiro
- Abstract
This manuscript elucidates the occurrence of glanders in an asymptomatic mare from Brazil presenting positive Burkholderia mallei antibody titers. The diagnosis was established through a multi-pronged approach encompassing microbiological culture, mass spectrometry, and genome sequencing. The outbreak occurred in 2019 in Tatuí, São Paulo, Brazil, and the infected mare, despite displaying no clinical symptoms, had multiple miliary lesions in the liver, as well as intense catarrhal discharge in the trachea. Samples were collected from various organs and subjected to bacterial isolation, molecular detection, and identification. The strain was identified as B. mallei using PCR and confirmed by MALDI-TOF mass spectrometry. Whole-genome sequencing revealed a genome size of 5.51 Mb with a GC content of 65.8%, 5871 genes (including 4 rRNA and 53 tRNA genes), and 5583 coding DNA sequences (CDSs). Additionally, 227 predicted pseudogenes were detected. In silico analysis of different genomic loci that allow for differentiation with Burkholderia pseudomallei confirmed the identity of the isolate as B. mallei, in addition to the characteristic genome size. The BAC 86/19 strain was identified as lineage 3, sublineage 2, which includes other strains from Brazil, India, and Iran. The genome sequencing of this strain provides valuable information that can be used to better understand the pathogen and its epidemiology, as well as to develop diagnostic tools for glanders.
- Subjects
BRAZIL; MICROBIAL cultures; MASS spectrometry; GENOME size; MARES; BURKHOLDERIA pseudomallei; NUCLEOTIDE sequencing; TRANSFER RNA; GENOMES
- Publication
Pathogens, 2023, Vol 12, Issue 10, p1250
- ISSN
2076-0817
- Publication type
Article
- DOI
10.3390/pathogens12101250