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- Title
Evaluation of a New Spike (S)-Protein-Based Commercial Immunoassay for the Detection of Anti-SARS-CoV-2 IgG.
- Authors
Eberhardt, Kirsten Alexandra; Dewald, Felix; Heger, Eva; Gieselmann, Lutz; Vanshylla, Kanika; Wirtz, Maike; Kleipass, Franziska; Johannis, Wibke; Schommers, Philipp; Gruell, Henning; Brensing, Karl August; Müller, Roman-Ulrich; Augustin, Max; Lehmann, Clara; Koch, Manuel; Klein, Florian; Di Cristanziano, Veronica
- Abstract
Background: The investigation of the antibody response to SARS-CoV-2 represents a key aspect in facing the COVID-19 pandemic. In the present study, we compared the new Immundiagnostik IDK® anti-SARS-CoV-2 S1 IgG assay with four widely-used commercial serological assays for the detection of antibodies targeting S (spike) and NC (nucleocapsid) proteins. Methods: Serum samples were taken from an unbiased group of convalescent patients and from a negative control group. Sample were simultaneously analyzed by the new Immundiagnostik IDK® anti-SARS-CoV-2 S1 IgG assay, by the DiaSorin LIAISON® SARS-CoV-2 S1/S2 IgG assay, and by the Euroimmun anti-SARS-CoV-2 S1 IgG ELISA. Antibodies binding NC were detected by the Abbott SARS-CoV-2 IgG assay and by the pan-immunoglobulin immunoassay Roche Elecsys® anti-SARS-CoV-2. Moreover, we investigated samples of a group of COVID-19 convalescent subjects that were primarily tested S1 IgG non-reactive. Samples were also tested by live virus and pseudovirus neutralization tests. Results: Overall, the IDK® anti-SARS-CoV-2 S1 IgG assay showed the highest sensitivity among the evaluated spike (S) protein-based assays. Additionally, the Immundiagnostik assay correlated well with serum-neutralizing activity. Conclusions: The novel IDK® anti-SARS-CoV-2 S1 IgG assay showed high sensitivity and specificity, representing a valid option for use in the routine diagnostic.
- Subjects
IMMUNOASSAY; COVID-19 pandemic; ANTIBODY formation; COVID-19; NEUTRALIZATION tests; IMMUNOGLOBULIN G; VIRAL antibodies
- Publication
Microorganisms, 2021, Vol 9, Issue 4, p733
- ISSN
2076-2607
- Publication type
Article
- DOI
10.3390/microorganisms9040733