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- Title
STUDY ON ANTICANCER EFFECT OF SYNTHETIC BIOGENIC SOURCE OF GERMACRENE A.
- Authors
Haiyan Cao; Zihui Li; Ruqi Li; Boyang Mao; Chen Liu; Yutong Li; Guowei Zhang
- Abstract
Objective: Based on obtaining germacrene A, which is the analogue of synthetic β-elemene, by means of synthetic biology, this experiment aims to explore the anti-tumor effect of germacrene A. Methods: The cell line used in this study was bladder cancer T24 cells. The morphological changes of bladder cancer T24 cells treated with germacrene A were observed by inverted microscope; the death of cancer cells was observed by trypan blue staining; cell viability was measured by the MTT assay and the inhibition rate of the cancer cells by the germacrene A was investigated. Based on the results obtained from the experiments, the anti-cancer effect of germacrene A derived from synthetic biology was analyzed. Results: After administration, the volume of T24 cells was significantly reduced. The shape of the T24 cells gradually changed from wedge-shaped and polygonal to round, and the T24 cells soon fell off, atrophied and disintegrated. After Tet blue staining, a large number of T24 cells after administration were observed to be dead; It was found by means of MTT assay that T24 cells were inhibited to varying degrees by the effects of different concentrations of germacrene A. Conclusion: The above experimental results show that the synthetic biological source of germacrene A has obvious inhibitory effect on bladder cancer T24 cells, and this effect has obvious time and concentration dependence.
- Subjects
ANTINEOPLASTIC agents; BIOGENIC amines; GERMACRENE; SYNTHETIC biology; BLADDER cancer
- Publication
Journal of Drug Delivery & Therapeutics, 2018, Vol 8, Issue 5, p421
- ISSN
2250-1177
- Publication type
Article
- DOI
10.22270/jddt.v8i5.1896