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- Title
Ex vivo monitoring of human cytomegalovirus-specific CD8+ T-cell responses using QuantiFERON<sup>®</sup>-CMV.
- Authors
Walker, S.; Fazou, C.; Crough, T.; Holdsworth, R.; Kiely, P.; Veale, M.; Bell, S.; Gailbraith, A.; McNeil, K.; Jones, S.; Khanna, R.
- Abstract
We have developed a novel diagnostic technology to monitor the human cytomegalovirus (HCMV)-specific CD8+ T-cell responses that is based on the detection of secreted interferon-gamma (IFN-γ) in the whole blood (referred to as QuantiFERON®-CMV). Evaluation of QuantiFERON®-CMV in healthy individuals revealed that this technology was at least as sensitive and with some HCMV epitopes more sensitive than the ELISPOT for detecting ex vivo IFN-γ. Results from QuantiFERON®-CMV assays showed 97% (36/37 individuals) agreement with the anti-HCMV serology test in healthy individuals. Furthermore, we also show that this technology can be used to assess HCMV-specific T-cell responses in transplant patients. This study shows that QuantiFERON®-CMV is a simple, reproducible, and reliable test for the detection of IFN-γ in response to HCMV CD8+ T-cell epitopes, and may be a valuable diagnostic test for the detection of HCMV infection and a useful clinical tool for monitoring the immune response in immunosuppressed patients during therapy.
- Subjects
ANTIVIRAL agents; EPITOPES; IMMUNOLOGY; ANTINEOPLASTIC agents; IMMUNE response; GLYCOPROTEINS; CYTOMEGALOVIRUS diseases; HERPESVIRUS diseases; CYTOMEGALIC inclusion disease; T cells
- Publication
Transplant Infectious Disease, 2007, Vol 9, Issue 2, p165
- ISSN
1398-2273
- Publication type
Article
- DOI
10.1111/j.1399-3062.2006.00199.x