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- Title
Cellular uptake of magnetic nanoparticles imaged and quantified by magnetic particle imaging.
- Authors
Paysen, Hendrik; Loewa, Norbert; Stach, Anke; Wells, James; Kosch, Olaf; Twamley, Shailey; Makowski, Marcus R.; Schaeffter, Tobias; Ludwig, Antje; Wiekhorst, Frank
- Abstract
Magnetic particle imaging (MPI) is a non-invasive, non-ionizing imaging technique for the visualization and quantification of magnetic nanoparticles (MNPs). The technique is especially suitable for cell imaging as it offers zero background contribution from the surrounding tissue, high sensitivity, and good spatial and temporal resolutions. Previous studies have demonstrated that the dynamic magnetic behaviour of MNPs changes during cellular binding and internalization. In this study, we demonstrate how this information is encoded in the MPI imaging signal. Through MPI imaging we are able to discriminate between free and cell-bound MNPs in reconstructed images. This technique was used to image and quantify the changes that occur in-vitro when free MNPs come into contact with cells and undergo cellular-uptake over time. The quantitative MPI results were verified by colorimetric measurements of the iron content. The results showed a mean relative difference between the MPI results and the reference method of 23.8% for the quantification of cell-bound MNPs. With this technique, the uptake of MNPs in cells can be imaged and quantified directly from the first MNP cell contact, providing information on the dynamics of cellular uptake.
- Subjects
MAGNETIC nanoparticles; MAGNETIC particle imaging; NONIONIZING radiation; CELL imaging; MAGNETIC properties of metals
- Publication
Scientific Reports, 2020, Vol 10, Issue 1, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/s41598-020-58853-3