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- Title
PROTECTIVE EFFECTS OF GRAPE SEED EXTRACT (PROANTHOCYANIDIN) AND COENZYME Q-10 IN MUSTARD (MECHLORETHAMINE) INDUCED OCULAR INJURY.
- Authors
Erdem, Uzeyir; Ilhan, Abdullah; Bayrak, Ziya; Yaman, Halil; Yaren, Hakan; Yıldırım, Ali O.; Eyi, Yusuf E.; Oztas, E.
- Abstract
Gulhane Military Medical Faculty, Ankara,Turkey 1: Ophthalmology, 2: Pharmeseutıcal Technology, 3: Biochemistry, 4: CBRN Defence, 5: Emergency Medicine,6: Histology PURPOSE: In this study, we aimed to investigate the therapeutic effects of proanthocyanidin (PAC) and Co Q-10 on mechlorethamine (MEC, one of the nitrogen mustard agents) induced ocular surface injury. METHODS: A total of 18 male spraque-dawley rats were divided into four groups: Control, MEC, MEC+PAC and MEC+CoQ10. Three groups received single dose of MEC (0.1 mg/5μL) distilled on the ocular surface. Control animals were given BSS (balanced saline solution) saline only. PAC (100 mg/kg) was administered by gavage feeding and CoQ10 (10 mg/kg) intraperitoneally 30 min after the application of MEC, and after the same doses of PAC and CoQ10 was given every day for a total of five doses. RESULTS: MEC application resulted in severe histopathological changes typically seen as acute alkaline burn of the ocular surface. PAC showed considerable protection against ocular damage. Pathologic changes like corneal epithelial defects, stromal edema and neovascularization, limbal lymphocyte infiltration, destruction of the muscle fibers and in the lid, deformation of the hair follicles on the eyelashes and loss of hypodermal connective tissue fibers were almost absent. On the other hand; protective effects of CoQ10 found limited when compared to PAC. CONCLUSION‟s: PAC showed considerable protection against ocular pathologic changes like corneal epithelial defects, stromal edema and neovascularization, destruction of the muscle fibers and in the lid, deformation of the hair follicles on the eyelashes and loss of hypodermal connective tissue fibers were remained almost totally intact. These results implicates that antioxidants, especially PAC, must be considered as a dietary supplement for specific populations such as soldiers under the risk of facing a chemical assault. Further studies must be made to find the optimum treatment modalities in practice.
- Publication
Balkan Military Medical Review, 2013, Vol 16, p118
- ISSN
1107-6275
- Publication type
Article