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- Title
The rice LysM receptor-like kinase Os CERK1 is required for the perception of short-chain chitin oligomers in arbuscular mycorrhizal signaling.
- Authors
Carotenuto, Gennaro; Chabaud, Mireille; Miyata, Kana; Capozzi, Martina; Takeda, Naoya; Kaku, Hanae; Shibuya, Naoto; Nakagawa, Tomomi; Barker, David G.; Genre, Andrea
- Abstract
The rice lysin-motif (LysM) receptor-like kinase Os CERK1 is now known to have a dual role in both pathogenic and symbiotic interactions. Following the recent discovery that the Oscerk1 mutant is unable to host arbuscular mycorrhizal ( AM) fungi, we have examined whether Os CERK1 is directly involved in the perception of the short-chain chitin oligomers (Myc- COs) identified in AM fungal exudates and shown to activate nuclear calcium (Ca2+) spiking in the rice root epidermis., An Oscerk1 knockout mutant expressing the cameleon NLS- YC2.60 was used to monitor nuclear Ca2+ signaling following root treatment with either crude fungal exudates or purified Myc- COs., Compared with wild-type rice, Ca2+ spiking responses to AM fungal elicitation were absent in root atrichoblasts of the Oscerk1 mutant. By contrast, rice lines mutated in Os CEBiP, encoding the LysM receptor-like protein which associates with Os CERK1 to perceive chitin elicitors of the host immune defense pathway, responded positively to Myc- COs., These findings provide direct evidence that the bi-functional Os CERK1 plays a central role in perceiving short-chain Myc- CO signals and activating the downstream conserved symbiotic signal transduction pathway.
- Subjects
LYSINS; KINASES; VESICULAR-arbuscular mycorrhizas; ENDOMYCORRHIZAS; CHITIN; OLIGOMERS
- Publication
New Phytologist, 2017, Vol 214, Issue 4, p1440
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/nph.14539