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- Title
Mitochondrial and Nuclear DNA Variants in Amyotrophic Lateral Sclerosis: Enrichment in the Mitochondrial Control Region and Sirtuin Pathway Genes in Spinal Cord Tissue.
- Authors
Cox, Sharon Natasha; Lo Giudice, Claudio; Lavecchia, Anna; Poeta, Maria Luana; Chiara, Matteo; Picardi, Ernesto; Pesole, Graziano
- Abstract
Amyotrophic Lateral Sclerosis (ALS) is a progressive disease with prevalent mitochondrial dysfunctions affecting both upper and lower motor neurons in the motor cortex, brainstem, and spinal cord. Despite mitochondria having their own genome (mtDNA), in humans, most mitochondrial genes are encoded by the nuclear genome (nDNA). Our study aimed to simultaneously screen for nDNA and mtDNA genomes to assess for specific variant enrichment in ALS compared to control tissues. Here, we analysed whole exome (WES) and whole genome (WGS) sequencing data from spinal cord tissues, respectively, of 6 and 12 human donors. A total of 31,257 and 301,241 variants in nuclear-encoded mitochondrial genes were identified from WES and WGS, respectively, while mtDNA reads accounted for 73 and 332 variants. Despite technical differences, both datasets consistently revealed a specific enrichment of variants in the mitochondrial Control Region (CR) and in several of these genes directly associated with mitochondrial dynamics or with Sirtuin pathway genes within ALS tissues. Overall, our data support the hypothesis of a variant burden in specific genes, highlighting potential actionable targets for therapeutic interventions in ALS.
- Subjects
MITOCHONDRIAL DNA; MOTOR neuron diseases; AMYOTROPHIC lateral sclerosis; NUCLEAR DNA; SPINAL cord; MITOCHONDRIA; MOTOR neurons; GENES
- Publication
Biomolecules (2218-273X), 2024, Vol 14, Issue 4, p411
- ISSN
2218-273X
- Publication type
Article
- DOI
10.3390/biom14040411