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- Title
Intact Long-Type dup A as a Marker for Gastroduodenal Diseases in Okinawan Subpopulation, Japan.
- Authors
Takahashi, Ayaka; Shiota, Seiji; Matsunari, Osamu; Watada, Masahide; Suzuki, Rumiko; Nakachi, Saori; Kinjo, Nagisa; Kinjo, Fukunori; Yamaoka, Yoshio
- Abstract
Background Helicobacter pylori dup A can be divided into two types according to the presence or absence of the mutation. In addition, full-sequenced data revealed that dup A has two types with different lengths depend on the presence of approximately 600 bp in the putative 5′ region (presence; long-type and absence; short-type), which has not been taken into account in previous studies. Methods A total of 319 strains isolated from Okinawa, the south islands of Japan, were included. The status of dup A and cag A was determined by polymerase chain reaction. The presence of mutations in long-type dup A was determined by DNA sequencing. Results The prevalence of long-type dup A was 26.3% (84/319). Sequence analysis showed that there were only six cases (7.1%) with point mutations lead to stop codon among 84 long-type dup A strains studied. Interestingly, intact long-type dup A without frameshift mutation , but not short-type dup A, was significantly associated with gastric ulcer and gastric cancer than gastritis ( p = .001 and p = .019, respectively). After adjustment by age, gender, and cag A, the presence of intact long-type dup A was significantly associated with gastric ulcer and gastric cancer compared with gastritis (odds ratio [ OR] = 3.35, 95% confidence interval [ CI] = 1.55-7.24 and OR = 4.14, 95% CI = 1.23-13.94, respectively). Conclusions Intact long-type dup A is a real virulence marker for severe outcomes in Okinawa, Japan. The previous information gained from PCR-based methods without taking long-type dup A into account must be interpreted with caution.
- Subjects
OKINAWA-shi (Japan); JAPAN; HELICOBACTER pylori; DUODENAL ulcers; GASTRIC mucosa; NUCLEOTIDE sequence; CANCER
- Publication
Helicobacter, 2013, Vol 18, Issue 1, p66
- ISSN
1083-4389
- Publication type
Article
- DOI
10.1111/j.1523-5378.2012.00994.x