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- Title
Differentiation of Clinical Helicobacter pullorum Isolates from RelatedHelicobacter andCampylobacter Species.
- Authors
Melito, Pasquale L.; Woodward, David L.; Bernard, Kathryn A.; Price, Lawrence; Khakhria, Rasik; Johnson, Wendy M.; Rodgers, Frank G.
- Abstract
Background: Helicobacter pullorum, first detected in the liver and intestinal contents of poultry, was defined as a new species in 1994. This organism has since been isolated from humans with gastroenteritis. Phenotypic as well as genotypic methods have been used to identify H. pullorum associated with cases of human disease. Materials and Methods: Clinical isolates were submitted for identification to the National Laboratory for Enteric Pathogens by Provincial Public Health Laboratories within Canada. Phenotypic characterization was conducted using a variety of growth and biochemical tests including oxidase, catalase, indoxyl acetate, H[sub 2]S production in triple sugar iron (TSI) agar, antimicrobial susceptibility testing, and fatty acid analysis. Genotypic identification was performed using a polymerase chain reaction–restriction fragment–length polymorphism (PCR-RFLP) analysis of a 1-kb fragment of the Helicobacter 16S rRNA gene. Results: During the last 7 years (1993–1999) a total of 11 isolates of H. pullorum were detected from patients with gastroenteritis for inclusion in this study. Typically, these isolates were oxidase and catalase positive, produced optimal growth at 42°C, and produced H[sub 2]S in TSI. Of these 11 isolates, 1 showed DNase activity, while another did not produce H[sub 2]S in TSI, and only 2 showed tolerance to 1% bile. Antimicrobial susceptibility assays indicated that 6 of the 11 strains were resistant to nalidixic acid. The fatty acid profiles of the isolates were similar to each other and provided a distinguishing profile from the other related species. Genetically identical and distinct species-specific restriction fragment–length polymorphism (RFLP) patterns were produced using the restriction enzymes Bsr I and Dde I. Conclusion: Phenotypic and genotypic procedures were used to identify H. pullorum. Interspecies phenotypic variability was apparent and supported the use of a polyphasic appr...
- Subjects
SPIRILLACEAE; MICROBIAL differentiation
- Publication
Helicobacter, 2000, Vol 5, Issue 3, p142
- ISSN
1083-4389
- Publication type
Article
- DOI
10.1046/j.1523-5378.2000.00022.x