We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Qualitative Proteome-Wide Analysis Reveals the Diverse Functions of Lysine Crotonylation in Dendrobium huoshanense.
- Authors
Wu, Jing; Meng, Xiaoxi; Jiang, Weimin; Wang, Zhaojian; Zhang, Jing; Meng, Fei; Yao, Xiaoyan; Ye, Mengjuan; Yao, Liang; Wang, Longhai; Yu, Nianjun; Peng, Daiyin; Xing, Shihai
- Abstract
The lysine crotonylation of histone proteins is a newly identified posttranslational modification with diversified cellular functions. However, there are few reports on lysine crotonylation of non-histone proteins in medicinal plant cells. By using high-resolution liquid chromatography–mass spectrometry (LC-MS) coupled with highly sensitive-specific immune-affinity antibody analysis, a whole crotonylation proteome analysis of Dendrobium huoshanense was performed. In total, 1,591 proteins with 4,726 lysine crotonylation sites were identified; among them, 11 conserved motifs were identified. Bioinformatic analyses linked crotonylated proteins to the drought stress response and multiple metabolic pathways, including secondary metabolite biosynthesis, transport and catabolism, energy production and conversion, carbohydrate transport and metabolism, translation, and ribosomal structure and biogenesis. This study contributes toward understanding the regulatory mechanism of polysaccharide biosynthesis at the crotonylation level even under abiotic stress. Outline of contents in the study.
- Subjects
LIQUID chromatography-mass spectrometry; LYSINE; PROTEOMICS; POST-translational modification; DENDROBIUM; HISTONES; SECONDARY metabolism; CARBOHYDRATE metabolism
- Publication
Frontiers in Plant Science, 2022, Vol 12, p1
- ISSN
1664-462X
- Publication type
Article
- DOI
10.3389/fpls.2022.822374