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- Title
P2X<sub>2</sub> and P2Y<sub>1</sub> immunofluorescence in rat neostriatal medium- spiny projection neurones and cholinergic interneurones is not linked to respective purinergic receptor function.
- Authors
Scheibler, Peter; Pesic, Mihail; Franke, Heike; Reinhardt, Robert; Wirkner, Kerstin; illes, Peter; Nörenberg, Wolfgang
- Abstract
1. The presence of ionotropic P2X receptors, targets of ATP in fast synaptic transmission, as well as metabotropic P2Y receptors, known to activate K+ currents in cultured neostriatal neurons, was invistifated in medium-spiny neutrons and cholinergic interneurones contained in neostriatal brain slices from 5-26-day-old rats. 2. In these cells, adenosine-5'-triphosphate (ATP) (100-1000 µM), 2-methylthioadenosine-5'-triphosphate (2MeSATP), α,β-methuleneadenosine-5'-triphosphate (α,βmeATP, 30-300 µM, each) and adenosine-5'-O-(3-thiotriphosphate (ATPγS) (100 µM) failed to evoke P2X receptor currents even when 8-cyclopentyl-1,3,-dipropylxanthine (DPCPX, 0.1 µM), apyrase (10 U ml-1) or intracellular Cs+ was used to prevent occluding effects of the ATP breakdown product adenosine, desensitization of P2X receptors by endogenous ATP and an interference with the activation of K+ channels, respectively. P2X receptor agonists were also ineffective in outside-out patches withdrawn from the brain slice tissue. Muscimol (10 µM) evoked GABAA receptor-mediated currents under all these conditions. 3. When used as a control, locus coeruleus neurons responded with P2X receptor-mediated currents for ATP (300 µM) each. 4. ATP and adenosine-5'-diphosphate (ADP) (100 µM, each) did not activate K+ currents in the neostriatal neutrons. 5. Despite the observed lack of function, P2X2 and P2Y1 immunofluoroscence was found in roughly 50% of the medium-spiny neurons and cholinergic interneutrones. 6. A role of ATP in synaptic transmission to striatal medium-spiny neutrons and cholinergic interneurones appears unlikely, however, the otherwise silent P2X and P2Y receptors may gain functionality under-certain yet unknown conditions.
- Subjects
NEURAL transmission; ADENOSINE triphosphate; NEURONS; PHARMACOLOGY; MEDICAL sciences
- Publication
British Journal of Pharmacology, 2004, Vol 143, Issue 1, p119
- ISSN
0007-1188
- Publication type
Article
- DOI
10.1038/sj.bjp.0705916