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- Title
descSPIM: an affordable and easy-to-build light-sheet microscope optimized for tissue clearing techniques.
- Authors
Otomo, Kohei; Omura, Takaki; Nozawa, Yuki; Edwards, Steven J.; Sato, Yukihiko; Saito, Yuri; Yagishita, Shigehiro; Uchida, Hitoshi; Watakabe, Yuki; Naitou, Kiyotada; Yanai, Rin; Sahara, Naruhiko; Takagi, Satoshi; Katayama, Ryohei; Iwata, Yusuke; Shiokawa, Toshiro; Hayakawa, Yoku; Otsuka, Kensuke; Watanabe-Takano, Haruko; Haneda, Yuka
- Abstract
Despite widespread adoption of tissue clearing techniques in recent years, poor access to suitable light-sheet fluorescence microscopes remains a major obstacle for biomedical end-users. Here, we present descSPIM (desktop-equipped SPIM for cleared specimens), a low-cost ($20,000–50,000), low-expertise (one-day installation by a non-expert), yet practical do-it-yourself light-sheet microscope as a solution for this bottleneck. Even the most fundamental configuration of descSPIM enables multi-color imaging of whole mouse brains and a cancer cell line-derived xenograft tumor mass for the visualization of neurocircuitry, assessment of drug distribution, and pathological examination by false-colored hematoxylin and eosin staining in a three-dimensional manner. Academically open-sourced (https://github.com/dbsb-juntendo/descSPIM), descSPIM allows routine three-dimensional imaging of cleared samples in minutes. Thus, the dissemination of descSPIM will accelerate biomedical discoveries driven by tissue clearing technologies. Poor access to suitable light-sheet microscopes remains a big obstacle for many end-users in biomedical research. Here, the authors present descSPIM, a low-cost, low-expertise light-sheet microscope for routine 3D imaging of cleared samples.
- Subjects
HEMATOXYLIN &; eosin staining; MICROSCOPES; THREE-dimensional imaging; BRAIN cancer
- Publication
Nature Communications, 2024, Vol 15, Issue 1, p1
- ISSN
2041-1723
- Publication type
Article
- DOI
10.1038/s41467-024-49131-1