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- Title
A rapid environmental DNA method for detecting white sharks in the open ocean.
- Authors
Truelove, Nathan K.; Andruszkiewicz, Elizabeth A.; Block, Barbara A.; Gilbert, M. Thomas P.
- Abstract
Environmental DNA (eDNA) research often requires returning to the laboratory for processing, which can delay species identification by weeks–months.Using a portable Oxford Nanopore MinIon sequencer, eDNA from white shark (Carcharodon carcharias) was rapidly identified from seawater samples collected in the high seas where they have historically been identified with biologging datasets.A total of 10 sequencing runs were performed on the MinIon onboard an oceanographic vessel with a turnaround time from water collection to sequence results and annotation of ~48 hr.Identifying vertebrates by amplifying eDNA from seawater provides a novel approach for sampling and detecting the presence of elusive species of conservation importance in remote locations.
- Subjects
WHITE shark; DNA; WILDLIFE conservation; OCEAN; TURNAROUND time
- Publication
Methods in Ecology & Evolution, 2019, Vol 10, Issue 8, p1128
- ISSN
2041-210X
- Publication type
Article
- DOI
10.1111/2041-210X.13201