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- Title
Potato Dextrose Agar With Rose-Bengal and Chloramphenicol: A New Culture Medium to Isolate Pathogenic Exophiala dermatitidis From the Environment.
- Authors
Jayaram, Mehalene; Hideyuki Nagao
- Abstract
Objective: The objective of this study is to describe the potato dextrose agar (PDA) with rose-Bengal and chloramphenicol as a new and simple medium (R-PDA chloramphenicol agar) to facilitate the detection of black yeast, Exophiala dermatitidis compared to ready-made conventional medium, namely rose- Bengal chloramphenicol agar. Methods: We prepared a new medium by adding chloramphenicol and rose-Bengal to ready-made PDA. Results: This medium proves better growth of the black yeast in terms of increased colony size compared to commercial rose- Bengal chloramphenicol agar. The increase in colony size aids for distinguishing the slow growing black yeast from all the other filamentous fungi. Conclusions: Compared to traditional rose-Bengal chloramphenicol agar, R-PDA chloramphenicol agar is superior to isolate Exophiala dermatitidis among other fast growing filamentous fungi which are present in the environment samples.
- Subjects
BACTERIAL growth; CHLORAMPHENICOL; CULTURE media (Biology); DYES &; dyeing; GLUCOSE; MICROBIAL ecology; MICROBIOLOGICAL techniques; YEAST
- Publication
Klimik Journal / Klimik Dergisi, 2018, Vol 31, Issue 1, p11
- ISSN
1301-143X
- Publication type
Article
- DOI
10.5152/kd.2018.05