We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Role of Ca<sup>2+</sup>, Calnexin and Calreticulin in Platelet from Adult Patients with Chronic Immune Thrombocytopenic Purpura.
- Authors
Xu, Da-Ming; Zhang, Ze-Wen; Yi, Jing-Xing; Xie, Long; Yu, Wen-Jun; Qiu, Jin-Feng; Xu, Cheng-Wei; He, Chun-Ling; Xu, Xian-Ru; Yin, Jun
- Abstract
Background: Adult chronic immune thrombocytopenia (chronic ITP) is a common autoimmune hemorrhagic disease characterized by decreased platelet production and increased platelet destruction, leading to thrombocytopenia. In this study, Ca2+, calnexin (CNX) and calreticulin (CRT) within platelets from adult patients with chronic ITP were investigated. Methods: Platelets were isolated from blood specimen collected from 20 adult patients with chronic ITP and 20 healthy volunteers. Ca2+, CNX and CRT were determined by flow cytometry, and the results were analyzed with EXPO32 ADC software. Results: Flow cytometry showed the expressions of Ca2+ (74.19± 19.40% vs 22.79± 10.47%) was elevated (P< 0.05). However, CNX (15.10± 7.32% vs 41.79± 14.45%) and CRT (25.11± 12.66% vs 38.58± 12.02%) were decreased markedly in platelets from adult patients with chronic ITP (P< 0.05 compared with healthy volunteers). Conclusion: Based on enhanced expression of Ca2+ and attenuated expression of CNX and CRT in patients with chronic ITP, Ca2+ concentration and its associated down-regulated proteins may be important regulatory signals in the pathogenesis of chronic ITP.
- Subjects
IDIOPATHIC thrombocytopenic purpura; CALRETICULIN; BLOOD platelets; HEMORRHAGIC diseases; ADULTS; FLOW cytometry
- Publication
International Journal of General Medicine, 2022, Vol 15, p2119
- ISSN
1178-7074
- Publication type
Article
- DOI
10.2147/IJGM.S347301