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- Title
Antibiotics resistance and molecular characterization of biofilm former bacteria, isolated from patients with chronic rhinosinusitis in Wasit Province, Iraq.
- Authors
Abdullah, Mohammed Abd; Abdulla, Abdul Basit Abdul Samad; Hameed, Husam Majeed
- Abstract
Background: Chronic rhinosinusitis (CRS) considered one of the common diseases, affecting nearly 10% of the general population, defined as an inflammatory disorder of the paranasal sinuses and linings of the nasal passages that last more than 12 weeks. The aim of the study is the determination of more predominant bacteria in patients' complaints from CRS in Wasit Province. Materials and Methods: One hundred and fifty patients were included in the present study with a case history of CRS. Isolated bacteria were identified by API system and confirmed by polymerase chain reaction (PCR) using speciesspecific primer pairs for 16S rRNA gene. Biofilm-related gene, icaA, icaD, and PpyR, was detected by PCR. Furthermore, antibiogram was performed according to the Clinical and Laboratory Standards Institute. Results: The results showed that 140 samples were positive for bacterial growth while only 10 were negative culture. The more predominant bacteria Staphylococcus aureus compile 41 (27.33%), 34 (22.66%) for Pseudomonas aeruginosa, 33(22%) for Staphylococcus epidermidis, 12 (8%) for Streptococcus pneumonia, 9 (6%) for Klebsiella pneumonia, 8 (5.33%) for Proteus mirabilis, 3 (2%) for Enterobacter aerogenes. According to gender, female was more affected than male, the age groups (15-24 years and 35-44 years) were the highest affected among others. Most of the infected patients were students. The biofilm-related genes were diagnosed among most three predominant species, icaA for S. aureus, icaD for S. epidermidis, and PpyR for P. aeruginosa. The results revealed that 38 (92.68%) of S. aureus, 33 (100%) of S. epidermidis, and 34 (100%) of P. aeruginosa have icaA, icaD, and PpyR, respectively. The highest resistance of isolates for antibiotics was S. aureus for erythromycin (82.92%), S. epidermidis for cefpodoxime (90.90%), S. pneumonia for erythromycin (41.66%), P. aeruginosa for cefpodoxime (100%), K. pneumonia for ceftazidime (100%), P. mirabilis for ceftriaxone (100%), and E. aerogenes for erythromycin (100%). The results revealed that the most effective antibiotics were ciprofloxacin and imipenem for all Gram-positive and Gram-negative bacteria. Conclusion: The study concludes that the predominant causative agents of CRS in Wasit Province were bacterial with capacity to form biofilm formation accompanied by antibiotic resistance.
- Subjects
IRAQ; DRUG resistance in bacteria; PSEUDOMONAS aeruginosa; ENTEROBACTER aerogenes; BACTERIA; GRAM-negative bacteria; SINUSITIS
- Publication
Drug Invention Today, 2020, Vol 14, Issue 2, p335
- ISSN
0975-7619
- Publication type
Article