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- Title
Anticancer potential of monosodium glutamate on KB cell lines.
- Authors
Sathvika, K.; Priya, V. Vishnu; Gayathri, R.
- Abstract
Introduction: Oral cancer is the third most common form of cancer in India and it is known to predominantly affect people on the lower end of the socioeconomic scale. Although there are various types of oral cancers, 90% of them are squamous cell carcinomas. Thus, we have attempted to test the anticancer potential of monosodium glutamate (MSG) - which is cheap and available as a part of our daily diet - through a 5-diphenyltetrazolium bromide (MTT) assay on KB cell lines. Materials and Methods: KB cell lines were procured and cultured in RPMI 1640 - with 10% fetal bovine serum (FBS), L-glutamine, 1% penicillin (100 U/mL), and streptomycin (100 µg/mL) at 37°C in a humidified chamber. A 96-well plastic culture plate was taken and each well was filled with 200 µL of the culture medium. They were then incubated at 37°C for 24 h to facilitate the attachment of the cells to the plates. MSG was purchased and dissolved in distilled water and made into different concentrations such as 50, 100, 150, and 200 µg/mL. Controls were included for each experimental setup. An MTT assay was performed to determine the percentage of cell viability, and the absorbance was read on an enzyme-linked immunosorbent assay reader at 570 nm. From the values obtained, the percentage of cytotoxicity (IC50 value) was calculated. Each experiment was carried out in triplicate, and the half maximal inhibitory concentration (IC50) of the test sample as the percentage survival of the cells was calculated. Results were expressed as mean ± SD. The statistical significance was determined by a oneway analysis of variance and post hoc least significant difference test. P < 0.05 was considered statistically significant. Results: MSG exhibits an anticancerous potential although it is lesser than that of the standard. MSG (200 mL) shows a moderate cytotoxicity while MSG (50 mL) hardly shows any anticancerous potential. Conclusion: Although moderate, MSG has a notable cytotoxicity that could possibly be used for treating oral cancer. Since MSG is readily available through our diet, it also presents as an easy and inexpensive solution for oral squamous cell carcinoma.
- Subjects
INDIA; MONOSODIUM glutamate; CELL lines; ENZYME-linked immunosorbent assay; ORAL cancer; SQUAMOUS cell carcinoma; INSULIN aspart
- Publication
Drug Invention Today, 2019, Vol 12, Issue 7, p1378
- ISSN
0975-7619
- Publication type
Article