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- Title
Age-Associated Changes in Interferon-γ and Interleukin-4 Secretion by Purified Human CD4+ and CD8+ T Cells.
- Authors
Yen, Chung-Jen; Lin, Shuei-Liong; Huang, Kuo-Tong; Lin, Rong-Hwa
- Abstract
Aging is associated with a decline in immune function. Interferon-γ (IFN-γ) and interleukin-4 (IL-4), two important immune deviation-related cytokines, are mainly produced by type 1 and type 2 T cells, respectively. To investigate the age-associated changes in the secretion of these two cytokines, 20 elderly and 20 young subjects fulfilling the SENIEUR protocol were enrolled. The ratios of CD4+ to CD8+ T cells were not different between the two age groups. The CD4+ and CD8+ T cells were purified by a magnetic cell sorting system, and then activated by concurrent anti-CD3 and anti-CD28 stimulation. The released cytokines were determined by ELISA. Both the CD4+ and the CD8+ T cells of the elderly individuals secreted a significantly larger amount of IFN-γ after activation. Profound IL-4 production by CD8+ T cells was observed in the older subjects compared with that of the young subjects. These data suggested that age-associated decrease in immunity may be related to an imbalance in the secretion of immune deviation cytokines. The number of IL-4-secreting CD8+ T cells (T cytotoxic 2) rose significantly in the older individuals. Our design also provided a useful way to differentiate the T cell subsets secreting the same cytokine, such as IFN-γ-producing T helper 1 and T cytotoxic 1 cells.Copyright © 2000 National Science Council, ROC and S. Karger AG, Basel
- Subjects
INTERFERONS; INTERLEUKIN-4; T cells; CYTOKINES; CELL-mediated cytotoxicity
- Publication
Journal of Biomedical Science, 2000, Vol 7, Issue 4, p317
- ISSN
1021-7770
- Publication type
Article
- DOI
10.1159/000025466