We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
RNA-SEQ: A GLANCE AT TECHNOLOGIES AND METHODOLOGIES.
- Authors
JAZAYERI, Seyed Mehdi; MELGAREJO MUÑOZ, Luz Marina; ROMERO, Hernán Mauricio
- Abstract
RNA Sequencing (RNA-Seq) is a newly born tool that has revolutionized the post-genomic era. The data produced by RNA-Seq, sequencing technologies and use of bioinformatics are exploding rapidly. In recent years, RNA-Seq has been the method of choice for profiling dynamic transcriptome taking advantage of high throughput sequencing technologies. RNA-Seq studies have shown the transcriptome magnitude, notion and complexity. From 2008, as its introduction year, the relevant reports on RNA-Seq have been multiplied by more than 2822 times just in 6 years. RNA-Seq also contributes a more accurate gene expression and transcript isoform estimation than other methods. Furthermore, some of the potential applications for RNA-Seq cannot be conducted by other methods and as yet are unique to RNA-Seq. As RNA-Seq approaches increase in speed and decrease in cost, more distinct researches are applied and become more common and accurate. RNA-Seq is a cross and interdisciplinary method that interconnects biology to other scientific topics. This article describes RNA-Seq approach, technologies, methodologies, implementation, and methods done so far in characterizing and profiling transcriptomes.
- Subjects
RNA sequencing; RNA analysis; NUCLEOTIDE analysis; NUCLEIC acid analysis; GENE expression in plants
- Publication
Acta Biológica Colombiana, 2015, Vol 20, Issue 2, p23
- ISSN
0120-548X
- Publication type
Article
- DOI
10.15446/abc.v20n2.43639