We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Mining a differential sialotranscriptome of Rhipicephalus microplus guides antigen discovery to formulate a vaccine that reduces tick infestations.
- Authors
Maruyama, Sandra R.; Garcia, Gustavo R.; Teixeira, Felipe R.; Brandão, Lucinda G.; Anderson, Jennifer M.; Ribeiro, José M. C.; Valenzuela, Jesus G.; Horackova, Jana; Veríssimo, Cecília J.; Katiki, Luciana M.; Banin, Tamy M.; Zangirolamo, Amanda F.; Gardinassi, Luiz G.; Ferreira, Beatriz R.; de Miranda-Santos, Isabel K. F.
- Abstract
Background: Ticks cause massive damage to livestock and vaccines are one sustainable substitute for the acaricides currently heavily used to control infestations. To guide antigen discovery for a vaccine that targets the gamut of parasitic strategies mediated by tick saliva and enables immunological memory, we exploited a transcriptome constructed from salivary glands from all stages of Rhipicephalus microplus ticks feeding on genetically tick-resistant and susceptible bovines. Results: Different levels of host anti-tick immunity affected gene expression in tick salivary glands; we thus selected four proteins encoded by genes weakly expressed in ticks attempting to feed on resistant hosts or otherwise abundantly expressed in ticks fed on susceptible hosts; these sialoproteins mediate four functions of parasitism deployed by male ticks and that do not induce antibodies in naturally infected, susceptible bovines. We then evaluated in tick-susceptible heifers an alum-adjuvanted vaccine formulated with recombinant proteins. Parasite performance (i.e. weight and numbers of females finishing their parasitic cycle) and titres of antigen-specific antibodies were significantly reduced or increased, respectively, in vaccinated versus control heifers, conferring an efficacy of 73.2%; two of the antigens were strong immunogens, rich in predicted T-cell epitopes and challenge infestations boosted antibody responses against them. Conclusion: Mining sialotranscriptomes guided by the immunity of tick-resistant hosts selected important targets and infestations boosted immune memory against salivary antigens.
- Subjects
RHIPICEPHALUS; TICK control; SALIVARY proteins; TRANSCRIPTION factors; VACCINES; ANTIGENS
- Publication
Parasites & Vectors, 2017, Vol 10, p1
- ISSN
1756-3305
- Publication type
Article
- DOI
10.1186/s13071-017-2136-2