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- Title
Detection of rabies vital antigens in non-autolysed and autolysed tissues by using an immunoperoxidase technique.
- Authors
Arslan, A.; Saglam, Y. S.; Temur, A.
- Abstract
The objectives of this study were to determine the potential of an immunoperoxidase technique involving the avidin-biotin complex (ABC) stain for the diagnosis of rabies in fresh tissues and compare it with other standard methods, including the fluorescent antibody test (FAT), haematoxylin and eosin and Seller's stain, and to investigate its capacity to detect rabies antigen in autolysed tissues. Samples of non-autolysed brain from 81 domestic and wild animals suspected of having rabies were examined. Rabies antigen was detected by FAT in 41 of these samples and Negri bodies were detected in 40 (97.6 per cent) of them by the immunoperoxidase technique, in 25 by haematoxylin and eosin and in 22 by Seller's stain. The sensitivity of the immunoperoxidase technique decreased as the tissues were left to autolyse; after two days it was 91.2 per cent, after four days 70.6 per cent, and after seven days 11.8 per cent.
- Subjects
RABIES; VIRUS diseases; SURGERY; DIAGNOSIS; MEDICAL care; CLINICAL medicine; ANIMALS
- Publication
Veterinary Record: Journal of the British Veterinary Association, 2004, Vol 155, Issue 18, p550
- ISSN
0042-4900
- Publication type
Article
- DOI
10.1136/vr.155.18.550