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- Title
Molecular bases of the antigens of the Lutheran blood group system.
- Authors
Crew, Vanja Karamatic; Green, Carole; Daniels, Geoff
- Abstract
Lutheran is a complex blood group system consisting of 18 identified antigens. There are four pairs of allelic antigens, whereas others are independently expressed antigens of a high frequency. Lutheran antigens are carried by the Lutheran glycoproteins, which are a product of a single gene LU. Genomic DNA from 21 individuals of 12 Lutheran phenotypes was used for PCR amplification of selected LU exons that were directly sequenced and compared to control DNA of a common Lutheran phenotype. Lutheran phenotypes were mostly caused by single-nucleotide polymorphisms within LU, resulting in single amino acid changes. The following mutations were observed: in LU:−4, G524A, Arg175Gln; in LU:−5, G326A, Arg109His; in LU:−6,9, C824T, Ser275Phe; in LU:−8,14, T611A, Met204Lys; in LU:−13, three point mutations (C1340T, Ser447Leu, C1671T silent mutation for Ser557 and A1742T, Gln581Leu); in LU:−16, C679T, Arg227Cys; in LU:−17, G340A, Glu114Lys; and in LU:−20, C905T, Thr302Met. Two LU:−12 samples had differing results: one individual had a deletion 99GCGCTT, Arg34 and Leu35, whereas the second LU:−12 sample had a point mutation G419A, Arg140Gln. The results revealed the genetic background of 11 Lutheran antigens and suggested their placement on the Lutheran glycoprotein.
- Subjects
ANTIGENS; PHENOTYPES; GENETIC polymorphisms; AMINO acids
- Publication
Transfusion, 2003, Vol 43, Issue 12, p1729
- ISSN
0041-1132
- Publication type
Article
- DOI
10.1111/j.0041-1132.2003.00600.x