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- Title
Regulation of IL-24 in human oral keratinocytes stimulated with Tannerella forsythia.
- Authors
Ko, Yeon‐Kyeong; An, Sun‐Jin; Han, Na‐Young; Lee, Hookeun; Choi, Bong‐Kyu; Ko, Yeon-Kyeong; An, Sun-Jin; Han, Na-Young; Choi, Bong-Kyu
- Abstract
Interleukin-24 is a pleiotropic immunoregulatory cytokine and a member of the IL-20R subfamily of the IL-10 family. The aim of this study was to investigate the regulation of IL-24 in the human oral keratinocyte cell line HOK-16B following infection with Tannerella forsythia, a major periodontal pathogen. T. forsythia induced the expression of IL-24 mRNA and the secretion of glycosylated IL-24 in HOK-16B cells. Glycosylation of IL-24 is linked to its solubility and bioavailability. T. forsythia-stimulated reactive oxygen species (ROS) induced the expression of IL-24, which was regulated by IL-6. The ROS inhibitor N-acetylcysteine and MAPK inhibitors significantly reduced the expression of IL-6 and IL-24 induced by T. forsythia. Recombinant human IL-24 significantly enhanced the expression of IL-1α, IL-8, CXCL10, and MCP-1 in HOK-16B cells. Together, these results indicate that ROS, MAPKs, and IL-6 comprise the axis of IL-24 expression in HOK-16B cells stimulated with T. forsythia. Thus, IL-24 may be involved in inflammation in oral keratinocytes.
- Subjects
INTERLEUKINS; FORSYTHIA; MESSENGER RNA; GLYCOSYLATION; MITOGEN-activated protein kinases; BIOAVAILABILITY
- Publication
Molecular Oral Microbiology, 2019, Vol 34, Issue 5, p209
- ISSN
2041-1006
- Publication type
journal article
- DOI
10.1111/omi.12265