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- Title
Switch from capsid protein import to adenovirus assembly by cleavage of nuclear transport signals.
- Authors
Harald Wodrich; Tinglu Guan; Gino Cingolani; Dan Von Seggern; Glen Nemerow; Larry Gerace
- Abstract
Replication and assembly of adenovirus occurs in the nucleus of infected cells, requiring the nuclear import of all viral structural proteins. In this report we show that nuclear import of the major capsid protein, hexon, is mediated by protein VI, a structural protein located underneath the 12 vertices of the adenoviral capsid. Our data indicate that protein VI shuttles between the nucleus and the cytoplasm and that it links hexon to the nuclear import machinery via an importin α/β-dependent mechanism. Key nuclear import and export signals of protein VI are located in a short C-terminal segment, which is proteolytically removed during virus maturation. The removal of these C-terminal transport signals appears to trigger a functional transition in protein VI, from a role in supporting hexon nuclear import to a structural role in virus assembly.
- Subjects
VIRAL replication; ADENOVIRUSES; PROTEINS; CYTOPLASM
- Publication
EMBO Journal, 2003, Vol 22, Issue 23, p6245
- ISSN
0261-4189
- Publication type
Article
- DOI
10.1093/emboj/cdg614