We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Direct visualization of specifically modified extracellular glycans in living animals.
- Authors
Attreed, Matthew; Desbois, Muriel; van Kuppevelt, Toin H; Bülow, Hannes E
- Abstract
Modification patterns of heparan sulfate coordinate protein function in metazoans, yet in vivo imaging of such non-genetically encoded structures has been impossible. Here we report a transgenic method in Caenorhabditis elegans that allows direct live imaging of specific heparan sulfate modification patterns. This experimental approach reveals a dynamic and cell-specific heparan sulfate landscape and could in principle be adapted to visualize and analyze any extracellular molecule in vivo.
- Subjects
HEPARAN sulfate; CAENORHABDITIS elegans; GLYCAN analysis; IMMUNOHISTOCHEMISTRY techniques; IMAGING systems in chemistry
- Publication
Nature Methods, 2012, Vol 9, Issue 5, p477
- ISSN
1548-7091
- Publication type
Article
- DOI
10.1038/nmeth.1945