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- Title
Evaluation of resistance to Pythium root rot by Pythium arrhenomanes in corn by using a toothpick inoculation method.
- Authors
Mitsuhashi, Shohei; Masunaka, Akira; Kikawada, Tomohiro; Sugawara, Koya; Tsukiboshi, Takao; Tamaki, Hiroyuki; Sato, Hisashi
- Abstract
A toothpick inoculation method was applied for evaluating the disease severity and infection frequency of Pythium root rot, which seriously restricts forage corn production in Japan. Seven corn hybrids were inoculated with toothpicks infested with Pythium arrhenomanes, the causal pathogen of the disease. Between 2011 and 2013, toothpicks were inserted into holes of the bottom stalks of corn plants just above the ground at the silking stage. At the inoculation plots throughout the experimental years, the infection frequencies of highly susceptible hybrids were stable even under suboptimal climate conditions. Therefore, we have concluded that the toothpick method is useful especially for discarding highly susceptible hybrids based on the data obtained from a single year. Further experiments will be required to overcome such problems as the low correlation between hybrids and their parental inbreds, and the development of more accurate screening systems for selecting resistant genotypes.
- Subjects
DISEASE resistance of plants; PYTHIUM diseases; CORN diseases; ROOT rots; CORN inoculation
- Publication
Grassland Science, 2015, Vol 61, Issue 3, p181
- ISSN
1744-6961
- Publication type
Article
- DOI
10.1111/grs.12096