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- Title
Ovalbumin-induced plasma interleukin-4 levels are reduced in ceramide kinase-deficient DO11.10 RAG1<sup>-/-</sup> mice.
- Authors
Niwa, Satoru; Urtz, Nicole; Baumruker, Thomas; Billich, Andreas; Bornancin, Frédéric
- Abstract
Ceramide kinase (CERK) produces the bioactive lipid ceramide-1-phosphate (C1P) and is a key regulator of ceramide and dihydroceramide levels. It is likely that CERK and C1P play a role in inflammatory processes but the cells involved and the mechanisms used remain to be clarified. In particular, the impact of CERK on T-cell biology has not been studied so far. Here, we used Cerk-/- mice backcrossed with DO11.10/RAG1-/- mice to probe the effect of CERK ablation on T-cell activation. Levels of interleukin (IL)-2, IL-4, IL-5, IL-13, of tumor necrosis factor (TNF)-a, and of interferon (INF)-g were recorded following ovalbumin challenge in vivo and using ovalbumin-treated splenocytes ex- vivo. Absence of CERK led to a significant decrease in the production of IL-4, thus suggesting that CERK may polarize T cells towards the TH2 cell subtype. However, the importance of CERK to TH2 cell biology will have to be investigated further because in a model of asthma, which is TH2-cell driven, Cerk-/- mice responded like wild-type animals.
- Subjects
CERAMIDES; LIPIDS; CYTOLOGY; GLYCOPROTEINS; INTERLEUKIN-4; CELLULAR mechanics; NECROSIS
- Publication
Lipids in Health & Disease, 2010, Vol 9, p1
- ISSN
1476-511X
- Publication type
Article
- DOI
10.1186/1476-511X-9-1