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- Title
Capillary Electrophoretic Profile of β<sub>2</sub>-Microglobulin Intermediate Associated With Hemodialysis.
- Authors
Motomiya, Yoshihiro; Uji, Yoshinori; Ando, Yukio
- Abstract
We previously identified an intermediate β2-microglobulin (I-β2m), which is an amyloidogenic β2m variant, via capillary electrophoresis (CE) and reported hemodialysis (HD)-associated variations in the serum concentrations of each β2m component, including that found in the rebound phase. Recent research has indicated that I-β2m can bind, via the SO3- moiety, with glycosaminoglycan or proteoglycan, which are major components of interstitial tissue. Because alterations in I-β2m are likely to be important in view of the possible accumulation of amyloidogenic precursor proteins in the interstitial space, we studied the I-β2m profile as related to HD. We used CE to determine the I-β2m profile both at the start and at the end of HD and during the rebound phase in 12 HD patients. We found both an unfolded β2m and a destructured I-β2m. More important, two peaks appeared in the rebound phase, one suggesting a refolding and one suggesting an irreversible destruction. Given that the intercompartmental transfer coefficient for β2m is 1.0, our results indicated concomitant processes occurring after HD: refolding of the β2m conformation and trapping of destructured I-β2m in the extravascular space. Because the trapping of destructured I-β2m supposedly leads to accumulation of β2m in the interstitial space, we have proposed a new concept-a 'shuttle' concept-for amyloid formation from β2m in the HD setting.
- Publication
Therapeutic Apheresis & Dialysis, 2012, Vol 16, Issue 4, p350
- ISSN
1744-9979
- Publication type
Article
- DOI
10.1111/j.1744-9987.2012.01069.x