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- Title
Single‐beam dual‐color alternate‐pathway two‐photon spectroscopy: Toward an optical toolbox for redox biology.
- Authors
Chebotarev, Artem S.; Lanin, Aleksandr A.; Raevskii, Roman I.; Kostyuk, Alexander I.; Smolyarova, Daria D.; Bilan, Dmitry S.; Savitskii, Ilya V.; Fedotov, Andrei B.; Belousov, Vsevolod V.; Zheltikov, Aleksei M.
- Abstract
We demonstrate a versatile single‐laser platform for single‐beam dual‐color two‐photon spectroscopy that combines a short‐pulse laser source with a tunable broadband wavelength converter based on a highly nonlinear photonic‐crystal fiber (PCF). We show that the short‐pulse PCF output can be tailored, via dispersion and nonlinearity management, to deliver a broadband optical driver whose spectral structure is ideally suited for a single‐beam two‐photon absorption (2PA) spectroscopy of the next‐generation genetically encodable fluorescent‐protein (FP) sensors of pH and redox‐responsive contrast agents. As a promising spectroscopic resource for redox biology, the short‐pulse PCF output can be finely sculpted to alternately drive an FP system via a one of its two 2PA‐allowed quantum pathways, yielding a high‐contrast fluorescence readout for a highly sensitive detection of redox reactions and signaling, pH sensing, and oxidative‐stress diagnosis.
- Subjects
OPTICAL spectroscopy; PHOTONIC crystal fibers; CONTRAST media; OXIDATION-reduction reaction; BIOLOGY; SINGLE photon generation
- Publication
Journal of Raman Spectroscopy, 2021, Vol 52, Issue 9, p1552
- ISSN
0377-0486
- Publication type
Article
- DOI
10.1002/jrs.6183