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- Title
Dynamic Volumetric Imaging of Mouse Cerebral Blood Vessels In Vivo with an Ultralong Anti-Diffracting Beam.
- Authors
Guo, Yong; Wang, Luwei; Luo, Ziyi; Zhu, Yinru; Gao, Xinwei; Weng, Xiaoyu; Wang, Yiping; Yan, Wei; Qu, Junle
- Abstract
Volumetric imaging of a mouse brain in vivo with one-photon and two-photon ultralong anti-diffracting (UAD) beam illumination was performed. The three-dimensional (3D) structure of blood vessels in the mouse brain were mapped to a two-dimensional (2D) image. The speed of volumetric imaging was significantly improved due to the long focal length of the UAD beam. Comparing one-photon and two-photon UAD beam volumetric imaging, we found that the imaging depth of two-photon volumetric imaging (80 μm) is better than that of one-photon volumetric imaging (60 μm), and the signal-to-background ratio (SBR) of two-photon volumetric imaging is two times that of one-photon volumetric imaging. Therefore, we used two-photon UAD volumetric imaging to perform dynamic volumetric imaging of mouse brain blood vessels in vivo, and obtained the blood flow velocity.
- Subjects
BLOOD vessels; FLOW velocity; BLOOD flow; MICE; FOCAL length; CEREBRAL angiography
- Publication
Molecules, 2023, Vol 28, Issue 13, p4936
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules28134936