We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Degradation of phenolic compounds by the lignocellulose deconstructing thermoacidophilic bacterium Alicyclobacillus Acidocaldarius.
- Authors
Aston, John; Apel, William; Lee, Brady; Thompson, David; Lacey, Jeffrey; Newby, Deborah; Reed, David.; Thompson, Vicki
- Abstract
Alicyclobacillus acidocaldarius, a thermoacidophilic bacterium, has a repertoire of thermo- and acid-stable enzymes that deconstruct lignocellulosic compounds. The work presented here describes the ability of A. acidocaldarius to reduce the concentration of the phenolic compounds: phenol, ferulic acid, ρ-coumaric acid and sinapinic acid during growth conditions. The extent and rate of the removal of these compounds were significantly increased by the presence of micro-molar copper concentrations, suggesting activity by copper oxidases that have been identified in the genome of A. acidocaldarius. Substrate removal kinetics was first order for phenol, ferulic acid, ρ-coumaric acid and sinapinic acid in the presence of 50 μM copper sulfate. In addition, laccase enzyme assays of cellular protein fractions suggested significant activity on a lignin analog between the temperatures of 45 and 90 °C. This work shows the potential for A. acidocaldarius to degrade phenolic compounds, demonstrating potential relevance to biofuel production and other industrial processes.
- Subjects
BIODEGRADATION; PHENOLS; LIGNOCELLULOSE; ACIDOPHILIC bacteria; BIOCHEMICAL substrates
- Publication
Journal of Industrial Microbiology & Biotechnology, 2016, Vol 43, Issue 1, p13
- ISSN
1367-5435
- Publication type
Article
- DOI
10.1007/s10295-015-1700-z