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- Title
小柴胡汤对大鼠慢性氟中毒致肝损伤的保护作用.
- Authors
陈伟杰; 吴浪浪; 彭玖燕; 梁超男; 贺廷贵; 吴楚珍; 唐文超
- Abstract
OBJECTIVE: To observe the therapeutic effects of Xiaochaihu decoction for liver injury induced by chronic fluorosis in rats, and to explore the protective effects of Xiaochaihu decoction on oxidative stress, so as to provide theoretical basis for the prevention and treatment of fluorosis. METHODS: SD rats were randomly divided into the blank control group, model group, Xiaochaihu decoction experimental group and Xiaochaihu decoction negative control group. The rat model of chronic fluorosis was established with NaF solution( 100 mg/L) as fluoride source. The Xiaochaihu decoction experimental group and the negative control group were given Xiaochaihu decoction( 7. 29 glkg ) by intragastric administration, while other groups were treated with 0. 9% sodium chloride solution by intragastric administration. After 90 d of continuous intragastric administration ( once a day ), the rats were anesthetized after fasting for 12 h, and the abdominal aorta blood was taken. The liver tissue was dissected under the ice bath, and the liver indicator was calculated. The pathological changes of liver tissue were detected by hematoxylin-eosin staining. Serum ALT and AST levels were detected by microplate method, liver MDA level was detected by thiobarbituric acid method, and liver T-SOD level was detected by microplate method. RESULTS: Compared with the blank control group, the levels of T-SO D, ALT, AST and MD A in the model group increased significantly, and the liver indicators increased significantly, with statistically significant differences ( P < 0. 01 ) . Pathological sections showed a large number of inflammatory infiltrations and dot necroses in the model group. Compared with the model group, the levels of ALT and AST in the Xiaochaihu decoction experimental group decreased(P<O. 05), the levels of MDA and T-SOD decreased significantly ( P < 0. 01 ), and the liver indicators decreased significantly ( P < 0. 01 ), with statistically significant differences. Pathological section results showed the pathological damage of liver in rats from Xiaochaihu decoction experimental group was improved to varying degrees, indicating that Xiaochaihu decoction could improve the pathological changes induced by liver injury in rats with chronic fluorosis. CONCLUSIONS : Xiaochaihu decoction has a certain alleviation effect on liver injury induced by chronic fluorosis, and its mechanism may be related to oxidative stress.
- Subjects
ABDOMINAL aorta; PATHOLOGICAL physiology; OXIDATIVE stress; LIVER injuries; FLUOROSIS; THERAPEUTIC alliance; PSYCHOTHERAPIST-patient relations
- Publication
Evaluation & Analysis of Drug-Use in Hospitals of China, 2022, Vol 22, Issue 3, p277
- ISSN
1672-2124
- Publication type
Article
- DOI
10.14009/j.issn.1672-2124.2022.03.005