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- Title
Identification and quantification of trehalose in vesicular-arbuscular mycorrhizal fungi by in vivo <sup>13</sup>C NMR and HPLC analyses.
- Authors
Bécard, G.; Doner, L. W.; Rolin, D. B.; Douds, D. D.; Pfeffer, P. E.
- Abstract
Natural abundance <em>in vivo</em> 12C NMR spectra were obtained from spores of three species of VA mycorrhizal fungi; <em>Glomus intraradix</em> Schenck & Smith, <em>Glomus etunicatum</em> Becker & Gerdemann and <em>Gigaspora margarita</em> Becker & Hall. The presence of trehalose was established in the spectra of <em>Gl. etunicatum</em> and <em>Gi. margarita</em>. HPLC analyses supported these observations and indicated that <em>Gl. intraradix</em> spores also contained small amounts of trehalose, not readily detectable by 13C NMR. Trehalose constituted between 0.06% and 1.6% (w/w) of the spore dry weight, depending on the species. Only trace amounts of other sugars, including glycerol, were detected. An <em>in vivo</em> NMR time course experiment indicated that trehalose was readily utilized during spore germination.
- Subjects
MYCORRHIZAS; PLANT spores; HIGH performance liquid chromatography; NUCLEAR magnetic resonance; SOIL fungi; ENDOGONACEAE
- Publication
New Phytologist, 1991, Vol 118, Issue 4, p547
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/j.1469-8137.1991.tb00994.x