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- Title
Quality and Fertilizing Ability In Vivo of Sex-Sorted Stallion Spermatozoa.
- Authors
Mari, G.; Rizzato, G.; Merlo, B.; Iacono, E.; Bucci, D.; Seren, E.; Tamanini, C.; Galeati, G.; Spinaci, M.
- Abstract
Contents Little information is available on the quality of stallion spermatozoa after sex sorting. The objectives of the present study were to assess the quality of sex-sorted stallion spermatozoa and determine its fertilizing ability after hysteroscopic low dose insemination. Ejaculates from four stallions were collected and sorted by a MoFlo SX® flow cytometer/sperm sorter. Before and after sorting, spermatozoa were evaluated for motility by Computer Assisted Sperm Analysis, viability (SYBR 14-propidium iodide), mitochondrial function (JC-1) and acrosomal status (fluorescein isothiocyanate Pisum sativum agglutinin conjugated). A fertility trial was carried out on four mares (seven oestrous cycles) by hysteroscopic insemination, depositing 5 × 106 X-bearing spermatozoa. Sex sorting resulted in a significant decrease (p < 0.001) in all motility characteristics. Sperm viability and percentage of spermatozoa with functional mitochondria were not affected by the sorting process, while the percentage of reacted spermatozoa was higher (p < 0.01) for non-sorted than sorted spermatozoa. Pregnancy rate was 28.6% (2/7) after low dose hysteroscopic insemination. Only one pregnancy was carried to term with the birth of a healthy filly. In conclusion, despite the reduction in sperm motility, sex sorting did not impair stallion sperm viability and mitochondrial activity immediately post-thaw; moreover, the sexed spermatozoa retained the ability to fertilize in vivo.
- Subjects
REPRODUCTION; SPERMATOZOA; FERTILITY; HORSE artificial insemination; MITOCHONDRIA
- Publication
Reproduction in Domestic Animals, 2010, Vol 45, Issue 2, p331
- ISSN
0936-6768
- Publication type
Article
- DOI
10.1111/j.1439-0531.2008.01314.x