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- Title
A role for the Salmonella Type III Secretion System 1 in bacterial adaptation to the cytosol of epithelial cells.
- Authors
Chong, Audrey; Starr, Tregei; Finn, Ciaran E.; Steele‐Mortimer, Olivia
- Abstract
Summary: Salmonella enterica serovar Typhimurium is a facultative intracellular pathogen that invades the intestinal epithelium. Following invasion of epithelial cells, Salmonella survives and replicates within two distinct intracellular niches. While all of the bacteria are initially taken up into a membrane bound vacuole, the Salmonella‐containing vacuole or SCV, a significant proportion of them promptly escape into the cytosol. Cytosolic Salmonella replicates more rapidly compared to the vacuolar population, although the reasons for this are not well understood. SipA, a multi‐function effector protein, has been shown to affect intracellular replication and is secreted by cytosolic Salmonella via the invasion‐associated Type III Secretion System 1 (T3SS1). Here, we have used a multipronged microscopy approach to show that SipA does not affect bacterial replication rates per se, but rather mediates intra‐cytosolic survival and/or initiation of replication following bacterial egress from the SCV. Altogether, our findings reveal an important role for SipA in the early survival of cytosolic Salmonella.
- Subjects
SECRETION; SALMONELLA enterica serovar typhimurium; BACTERIAL adaptation; EPITHELIAL cells; SALMONELLA; SALMONELLA enterica; CYTOSOL; INTRACELLULAR pathogens
- Publication
Molecular Microbiology, 2019, Vol 112, Issue 4, p1270
- ISSN
0950-382X
- Publication type
Article
- DOI
10.1111/mmi.14361