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- Title
Analysis of ginsenoside content, functional genes involved in ginsenosides biosynthesis, and activities of antioxidant enzymes in Panax quinquefolium L. adventitious roots by fungal elicitors.
- Authors
Li, Jinxin; Li, Hongfa; Liu, Dahui; Liu, Shujie; Li, Jianli; Wang, Juan
- Abstract
In this study, Candida tropicalis, Aspergillus niger, and a mixture of their cultures were previously used as fungal elicitors in Panax quinquefolium adventitious root culture to enhance ginsenoside production. The result showed that ginsenoside content (11.47 ± 0.54 mg g) was significantly enhanced to 3.25-fold of the control group after treatment with 400 mg L C. tropicalis. The polysaccharide content (213.01 ± 18.63 mg g) reached the highest when A. niger was at the concentration of 200 mg L. HPLC-ESI-MS analysis was performed, showing that ginsenosides Rh and Ro were identified after treatment with fungal elicitors. Furthermore, we found that C. tropicalis, A. niger, and mix elicitor significantly up-regulated the expression of the squalene synthase, squalene epoxidase, dammarenediol synthase, β-amyrin synthase, protopanaxadiol synthase, protopanaxatriol synthase, and UDP-glycosyltransferase. SDS-polyacrylamide gel electrophoresis was used to analysis the changes of protein. A new band was discovered at the size of 75 kDa. In addition, it found that fungal elicitors significantly increased the activities of catalases and peroxidase.
- Subjects
GINSENOSIDES; BIOSYNTHESIS; ANTIOXIDANTS; AMERICAN ginseng; ELICITORS (Botany); PLANT roots
- Publication
Research on Chemical Intermediates, 2017, Vol 43, Issue 4, p2415
- ISSN
0922-6168
- Publication type
Article
- DOI
10.1007/s11164-016-2770-x