We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Phenolic profile of a Parma violet unveiled by chemical and fluorescence imaging.
- Authors
Khatib, Moustafa; Pouzet, Cécile; Lafitte, Claude; Chervin, Justine; Bonzon-Ponnet, Valérie; Jauneau, Alain; Esquerré-Tugayé, Marie-Thérèse
- Abstract
The ability of phenolic compounds to autofluoresce upon illumination by UV or blue light was exploited to explore the nature and distribution of these metabolites within the flower petals, leaves and roots of the violet, Viola alba subsp. dehnhardtii. This was achieved through a dual complementary approach that combined fluorescence microscopy imaging of living intact tissues and chemical extraction of pulverized material. The blue to red fluorescence displayed by living tissues upon illumination was indicative of their richness in phenolic compounds. Phenolic acids were found in all tissues, while flavonoids characterized the aerial part of the plant, anthocyanidins being restricted to the petals. The chemical quantification of phenolics in plant extracts confirmed their tissue-specific distribution and abundance. A key finding was that the spectral signatures obtained through confocal microscopy of endogenous fluorophores in living tissues and their counterpart extracts share the same fluorescence patterns, pointing out the potential of fluorescence imaging of intact organs for a proper estimation of their phenolic content. In addition, this study highlighted a few distinct morphology cell types, in particular foliar-glandular-like structures, and jagged petal cell walls. Altogether, these data provide a comprehensive histochemical localization of phenolics in living tissues of a violet. Converting fluorescence imaging into a chemical imprint indicated that one can rely on fluorescence microscopy of intact living tissues as a rapid, non-destructive means to follow their phenolic imprint under various environmental conditions.
- Subjects
PARMA (Italy); FLUORESCENCE; FLUORESCENCE microscopy; FLOWER petals; CONFOCAL microscopy; PHENOLS
- Publication
AoB Plants, 2021, Vol 13, Issue 4, p1
- ISSN
2041-2851
- Publication type
Article
- DOI
10.1093/aobpla/plab041