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- Title
Nuclear DNA Modal Patterns as Diagnostic Criteria in Human Lung Cancer.
- Authors
OZAKI, HIROKI S.; YONEYAMA, TAKESHI; EGUCHI, KENJI; KODAMA, TETSURO; HOSHINO, KAZUMASA
- Abstract
In a prospective study of human lung cancer, the nuclear DNA content of tumor populations on 76 touch smears prepared from primary and metastatic lesions of 70 patients was determined cytofluorometrically. A modal DNA value was calculated from the distribution of the logarithmically transformed DNA content for each tumor. By the analysis of levels of modal DNA values, the nuclear DNA distribution histograms in lung tumors could be divided into the following three major types: diploid-tetraploid type (Type I), triploid type (Type II), and mixed type (Type III). Modal DNA values in Type I histograms were diploid (2c), tetraploid (4c), and octaploid (8c), whereas the modal DNA values in Type II histograms were near-triploid (3c), near-hexaploid (6c), and decaploid (10c). Type III histograms were characterized by the simultaneous existence of multiple modal DNA values at diploidy or tetraploidy and near-triploidy or near-hexaploidy. Each of the three major types was further divided into six, four, and four kinds of DNA histogram subtypes, respectively, according to modal DNA values. The most frequent DNA distribution pattern was the subtype A of the triploid type (Type IIA). Four of 14 DNA histogram subtypes were all of polyploid distribution patterns in which the relative DNA values in the individual modes differ from each other by a factor of two. This suggests that endoamitosis accounts for such polyploidy. In one of five patients whose primary and metastatic lesions were examined simultaneously, the DNA distribution pattern changed from Type IIA in the primary tumor to Type IIB in its metastasis. The diagnostic and prognostic implications of the 14 DNA histogram subtypes analyzed are discussed.
- Publication
Japanese Journal of Clinical Oncology, 1981, Vol 11, Issue 3, p431
- ISSN
0368-2811
- Publication type
Article